Hydrozoan Per Oxide (H2O2) Test

Hydrozoan Per Oxide (H₂O₂) Test

Make necessary solutions :

v  Potassium per manganite solution :

Process :

1.58 gm Potassium permanganate (KMnO₄) + 0.5 Litter Water (H₂O) for make 0.1 Normal solution

In 0.5 Litter volume metric flax. ( 3.16 KMnO₄ for 1 Litter H₂O)

This solution will be lasting 6 months but It will be covered by a black fabric/paper.

v  Sulfuric acid solution :

Process :

It will be make 10% H₂SO₄ Solution.

Such as : 25 ml H₂SO₄ + 225 ml H₂O

               50 ml H₂SO₄ + 450 ml H₂O

v  Per-Oxide solution :

Process :

1.25 gm (Liquid) H₂O₂ + 250 ml H₂O (5 gm/litter)

(Total volume will be 250 ml so water will less than 250 ml )

The solution will make in 250 ml volume metric flax.

Stapes :

1.      1.25 gm H₂O₂  will be taken by electric balance .

2.      Than it will be transfer in 250 ml V.F

3.      Than water will taken in flax border line so total volume will 250 ml

Test process :

1.     H₂O₂ Solution 25 ml + H₂SO₄ Solution 25 ml in a konical flax. (0.125 gm H₂O₂  in total solution)

2.     Burette will be fill by KMnO₄ Solution .

3.     KMnO₄ Solution will added in (H₂O₂+ H₂SO₄ ) solution by drop and drop and flax will shak.

4.     After change the (H₂O₂+ H₂SO₄ ) solution color (Slid pink) Burette will be stop.

5.     And burette reading will be count. Example . 28 ml

Calculation

Formula :

                                                               = 51.68 %

But 0.1 Normal solution’s actual Normality 0.095 N

So correct purity is

 = 49.096 %

NB:-Wight of sample  = (25 ml* 5 gm) / 1000 ml                                                         

                                     = 0.125 gm

          Meqt. Of H₂O₂   = 34 amu / 2000

                                    = 0.017